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What factors affect the quality of image obtained using a light microscope?

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What factors affect the quality of image obtained using a light microscope?

Post by biologyisg8 on Sat Jan 10, 2009 11:17 am

What factors affect the quality of image obtained using a light microscope? How can staining techniques be used to enhance the quality of the image?

Really want to know the answer from professionals like people in this forum. Answers highly appreciated. Thanks .

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Re: What factors affect the quality of image obtained using a light microscope?

Post by admin @ shivam on Sat Jan 10, 2009 11:25 am

It would help if you were to copy the precise question here. I feel it is a vague question to begin with. All steps prior to microscopy you do have to be optimized to get the best image.

0. Proper section thickness (ideally between 2 and 7 micron thickness).
1. Optimized fixation
2. Optimized staining
3. Use of counter-stains (for example, if you are looking at cytoplasm, also counter-stain nuclei)
4. Proper mounting (no bubbles, clean coverslips etc).
5. Proper storage.

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Great work !!

Post by Guru on Mon Jan 12, 2009 7:48 pm

admin @ shivam wrote:It would help if you were to copy the precise question here. I feel it is a vague question to begin with. All steps prior to microscopy you do have to be optimized to get the best image.

0. Proper section thickness (ideally between 2 and 7 micron thickness).
1. Optimized fixation
2. Optimized staining
3. Use of counter-stains (for example, if you are looking at cytoplasm, also counter-stain nuclei)
4. Proper mounting (no bubbles, clean coverslips etc).
5. Proper storage.

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ADMIN U DOING GREAT WORK

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Re : Great Work !!

Post by admin @ shivam on Mon Jan 12, 2009 7:50 pm

Thanks !!

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Good keep it up !!

Post by good boy on Tue Jan 13, 2009 8:28 pm

Admin u did g8 and fast and realible reply


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Agreed

Post by biologyisg8 on Wed Jan 14, 2009 11:04 am

ya ! I agree with u .......: Good Boy

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Thanks for your comments on me !

Post by admin @ shivam on Sat Feb 14, 2009 7:33 pm

Thanks !!!!!!!

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good

Post by J on Sun Feb 22, 2009 5:20 pm

gòód

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Thanks

Post by biologyisg8 on Fri Feb 27, 2009 8:30 pm

J wrote:gòód


Thanks for repling
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Re: What factors affect the quality of image obtained using a light microscope?

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