| What factors affect the quality of image obtained using a light microscope? | |
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biologyisg8 Member
Number of posts : 60 Age : 40 Location : Alienland Points : 55970 Reputation : 10 Registration date : 2009-01-10
| Subject: What factors affect the quality of image obtained using a light microscope? Sat Jan 10, 2009 11:17 am | |
| What factors affect the quality of image obtained using a light microscope? How can staining techniques be used to enhance the quality of the image? Really want to know the answer from professionals like people in this forum. Answers highly appreciated. Thanks . | |
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admin @ shivam Admin
Number of posts : 254 Age : 29 Location : Bareilly , UP Points : 56020 Reputation : 20 Registration date : 2009-01-09
| Subject: Re: What factors affect the quality of image obtained using a light microscope? Sat Jan 10, 2009 11:25 am | |
| It would help if you were to copy the precise question here. I feel it is a vague question to begin with. All steps prior to microscopy you do have to be optimized to get the best image. 0. Proper section thickness (ideally between 2 and 7 micron thickness). 1. Optimized fixation 2. Optimized staining 3. Use of counter-stains (for example, if you are looking at cytoplasm, also counter-stain nuclei) 4. Proper mounting (no bubbles, clean coverslips etc). 5. Proper storage. | |
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Guru Member
Number of posts : 2 Points : 55940 Reputation : 0 Registration date : 2009-01-12
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admin @ shivam Admin
Number of posts : 254 Age : 29 Location : Bareilly , UP Points : 56020 Reputation : 20 Registration date : 2009-01-09
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good boy Premium Mamber
Number of posts : 24 Points : 55940 Reputation : 10 Registration date : 2009-01-13
| Subject: Good keep it up !! Tue Jan 13, 2009 8:28 pm | |
| Admin u did g8 and fast and realible reply | |
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biologyisg8 Member
Number of posts : 60 Age : 40 Location : Alienland Points : 55970 Reputation : 10 Registration date : 2009-01-10
| Subject: Agreed Wed Jan 14, 2009 11:04 am | |
| ya ! I agree with u .......: Good Boy | |
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admin @ shivam Admin
Number of posts : 254 Age : 29 Location : Bareilly , UP Points : 56020 Reputation : 20 Registration date : 2009-01-09
| Subject: Thanks for your comments on me ! Sat Feb 14, 2009 7:33 pm | |
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J Member
Number of posts : 1 Points : 55530 Reputation : 0 Registration date : 2009-02-22
| Subject: good Sun Feb 22, 2009 5:20 pm | |
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biologyisg8 Member
Number of posts : 60 Age : 40 Location : Alienland Points : 55970 Reputation : 10 Registration date : 2009-01-10
| Subject: Thanks Fri Feb 27, 2009 8:30 pm | |
| - J wrote:
- gòód
Thanks for repling | |
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| Subject: Re: What factors affect the quality of image obtained using a light microscope? | |
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| What factors affect the quality of image obtained using a light microscope? | |
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