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What factors affect the quality of image obtained using a light microscope?

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The Great 21st century world :: Biology :: Cell and molecular biology

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What factors affect the quality of image obtained using a light microscope? Empty What factors affect the quality of image obtained using a light microscope?

Post by biologyisg8 Sat Jan 10, 2009 11:17 am

What factors affect the quality of image obtained using a light microscope? How can staining techniques be used to enhance the quality of the image?

Really want to know the answer from professionals like people in this forum. Answers highly appreciated. Thanks .

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What factors affect the quality of image obtained using a light microscope? Empty Re: What factors affect the quality of image obtained using a light microscope?

Post by admin @ shivam Sat Jan 10, 2009 11:25 am

It would help if you were to copy the precise question here. I feel it is a vague question to begin with. All steps prior to microscopy you do have to be optimized to get the best image.

0. Proper section thickness (ideally between 2 and 7 micron thickness).
1. Optimized fixation
2. Optimized staining
3. Use of counter-stains (for example, if you are looking at cytoplasm, also counter-stain nuclei)
4. Proper mounting (no bubbles, clean coverslips etc).
5. Proper storage.

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What factors affect the quality of image obtained using a light microscope? Empty Great work !!

Post by Guru Mon Jan 12, 2009 7:48 pm

admin @ shivam wrote:It would help if you were to copy the precise question here. I feel it is a vague question to begin with. All steps prior to microscopy you do have to be optimized to get the best image.

0. Proper section thickness (ideally between 2 and 7 micron thickness).
1. Optimized fixation
2. Optimized staining
3. Use of counter-stains (for example, if you are looking at cytoplasm, also counter-stain nuclei)
4. Proper mounting (no bubbles, clean coverslips etc).
5. Proper storage.

Basketball


ADMIN U DOING GREAT WORK
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What factors affect the quality of image obtained using a light microscope? Empty Re : Great Work !!

Post by admin @ shivam Mon Jan 12, 2009 7:50 pm

Thanks !!

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What factors affect the quality of image obtained using a light microscope? Empty Good keep it up !!

Post by good boy Tue Jan 13, 2009 8:28 pm

Admin u did g8 and fast and realible reply


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What factors affect the quality of image obtained using a light microscope? Empty Agreed

Post by biologyisg8 Wed Jan 14, 2009 11:04 am

ya ! I agree with u .......: Good Boy

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What factors affect the quality of image obtained using a light microscope? Empty Thanks for your comments on me !

Post by admin @ shivam Sat Feb 14, 2009 7:33 pm

Thanks !!!!!!!
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What factors affect the quality of image obtained using a light microscope? Empty good

Post by J Sun Feb 22, 2009 5:20 pm

gòód
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What factors affect the quality of image obtained using a light microscope? Empty Thanks

Post by biologyisg8 Fri Feb 27, 2009 8:30 pm

J wrote:gòód


Thanks for repling
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